个人简介

和玉兵，男，博士，副教授，博士生导师，中国农业科学院作物科学研究所“青年英才”。2010年本科毕业于华中农业大学生命科学技术学院国家理科基地班-生物技术，2017年博士毕业于华中农业大学生命科学技术学院，获孟山都奖学金、陶氏奖学金。2018年在赵云德教授实验室开展生长素及生物技术相关博士后研究，2019年作为高层次人才受聘于南京农业大学。主要研究成果：克隆并解析了第一个柱头完全退化的水稻雌性不育突变体并有望用于高效杂交制种，建立了水稻中的高效CRISPR/Cas9基因编辑系统，率先开发了转基因自杀基因编辑技术（TKC）和颜色辅助筛选等多种转基因清除的基因编辑方案，率先开发了可见光下裸眼可视的新型报告系统（RUBY），相关解决方已被国内外千余家实验室用于相关研究。主持国家级项目等7项，获得国家发明专利2项，以第一或通讯作者在Molecular plant、Plant Biotechnology Journal、Plant Physiology等学术期刊发表相关论文十余篇。2022年入选中国农业科学院青年英才计划引进工程所级人才

研究方向

基因组编辑技术开发与创新利用

主要科研项目

1.  引导编辑介导的水稻定点长同源片段替换体系构建与应用，国家自然科学基金青年科学基金项目，主持，在研；

2.  引导编辑介导的水稻大片段精准基因替换和插入，中国农业科学院青年创新专项，主持，在研；

3.  利用功能模块组装开发新型精准基因组编辑器，中国农业科学院南繁专项，主持，在研。

代表性论文（著）

1.  He Y, Zhu M, Wang L, Wu J, Wang Q, Wang R, Zhao Y*. Programmed Self-Elimination of the CRISPR/Cas9 Construct Greatly Accelerates the Isolation of Edited and Transgene-Free Rice Plants.Molecular Plant, 2018, 11(9): 1210-1213.

2.  He Y*#, Zhang T#, Sun H, Zhan H, Zhao Y*. A reporter for noninvasively monitoring gene expression and plant transformation.Horticulture Research, 2020, 7: 152. (* corresponding author, # co-first author)

3.  Yang N#, Yan L#, Zheng Z, Zhang Y, Zhan H, Tian Y, Zhang T, Li R, Gong X, Xu M, Zhu M, Wu J,He Y*, Zhao Y. Editing gene families by CRISPR/Cas9: accelerating the isolation of multiple transgene-free null mutant combinations with much reduced labor-intensive analysis.Plant Biotechnology Journal, 2022, 20(2): 241-243.

4.  He Y, Mudgett M, Zhao Y*. Advances in gene editing without residual transgenes in plants.Plant Physiology, 2022, 188(4): 1757-1768.

5.  LiuJL, Chen MM, Chen WQ, Liu CM,He Y†, Song XF†*. A CASE toolkit for easy and efficient multiplex transgene-free gene-editing.Plant Physiology, 2022, 188(4): 1843-1847.(† Senior author, * corresponding author)

6.  He Y, Yan L, Ge C, Yao XF, Han X, Wang R, Xiong L, Jiang L, Liu CM, Zhao Y*. PINOID Is Required for Formation of the Stigma and Style in Rice.Plant Physiology, 2019, 180(2): 926-936.

7.  He Y, Zhu M, Wang L, Wu J, Wang Q, Wang R, Zhao Y*. Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants.Rice Science, 2019, 26(2): 109-117.

8.  He Y*#, Zhu M#, Wu J#, Ouyang L, Wang R, Sun H, Yan L, Wang L, Xu M, Zhan H, Zhao Y. Repurposing of anthocyanin biosynthesis for plant transformation and genome editing.Frontiers in Genome Editing, 2020, 2: 607982.

9.  He Y, Zhao Y*. Technological breakthroughs in generating transgene-free and genetically stable CRISPR-edited plants.aBIOTECH, 2020, 1(1): 88-96.

10.  He Y, Zhang T, Yang N, Xu M, Yan L, Wang L, Wang R, Zhao Y*. Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing.Journal of Genetics and Genomics, 2017, 44(9): 469-472.

11.  He Y, Wang R, Dai X, Zhao Y*. On Improving CRISPR for Editing Plant Genes: Ribozyme-Mediated Guide RNA Production and Fluorescence-Based Technology for Isolating Transgene-Free Mutants Generated by CRISPR.Progress in Molecular Biology and Translational Science, 2017, 149: 151-166.

12.  He Y, Zhao Y*. A key link between jasmonic acid signaling and auxin biosynthesis.Science China Life Sciences, 2015, 58(3): 311-312.

13.  Wang Y, Wei S,He Y, Yan L, Wang R, Zhao Y*. Synergistic roles of LAX1 and FZP in the development of rice sterile lemma.Crop Journal, 2020, 8(1): 16-25.

14.  Li S, Li J,He Y, Xu M, Zhang J, Du W, Zhao Y*, Xia L*. Precise gene replacement in rice by RNA transcript-templated homologous recombination.Nature Biotechnology, 2019, 37(4): 445-450.

15.  Sun Y, Zhang X, Wu C,He Y, Ma Y, Hou H, Guo X, Du W, Zhao Y*, Xia L*. Engineering Herbicide-Resistant Rice Plants through CRISPR/Cas9-Mediated Homologous Recombination of Acetolactate Synthase.Molecular Plant, 2016, 9(4): 628-631.